VNUR20280 Laboratory Work for Veterinary Nurses

Academic Year 2022/2023

In this module the student will be introduced to the basic principles of clinical pathology, which is comprised of 3 subdisciplines, a) haematology, including haemostasis; b) clinical biochemistry, including endocrinology, molecular diagnostics, urinalysis, c) cytology, including microscopy and image analysis. The student will explore the theory and practice of sample preparation and a number of diagnostic tests in each of these subdisciplines. The student will learn the principles of laboratory work including laboratory safety, laboratory management, and quality control. The student will learn, practice, and perform many of the diagnostic tests of clinical pathology that are used in clinical practice. Attendance at practicals is compulsory as they are essential in order to fulfil the learning outcomes of this module. Students who fail to attend practicals will not be permitted to pass this module.

Show/hide contentOpenClose All

Curricular information is subject to change

Learning Outcomes:

On successful completion of this module, the student will be able to:
1. Discuss commonly used laboratory tests, their indications and basic interpretation.
2. Outline laboratory safety, laboratory management, and quality control measures.
3. Demonstrate knowledge and performance of a range of practical laboratory skills relevant to clinical pathology

Indicative Module Content:

Lectures, Tutorials, and Practicals: Titles and Content -
1) Introduction to module and to ClinPath -lectures Module description. Objectives. Assessment details. Schedule. ClinPath texts and references. Definitions. Sub-disciplines of ClinPath. Description, components of, and example of a clinpath profile. Lab management. Pre-analytical vs analytical variation. Blood collection. Serum versus plasma
2) Working in the ClinPath Lab - lectures Practice vs Referral labs. Submissions to referral labs. Setting up a clinpath area in a vet practice. Health and Safety. PPE, MSDS, Waste disposal. Emergencies in the lab. First Aid. Safe behavior. Basic equipment in the lab area of the vet practice. Laboratory information management systems. Internal and external QC, GLP, SOP, validation
3) The RBC - lectures Definitions. The CBC. Determining and interpreting haematocrit with total protein. Determining and interpreting RBC indices. Making, drying, staining and examining blood smears. Abnormal erythrocyte shapes. Common findings in anaemias: immune-mediated, infectious anaemia, Heinz body
4) The RBC - tutorials Discussion and illustration of the standard operating procedures to be followed in the lab on RBCs
5) The RBC - practicals Demo then supervised performance by each student: measurement / interpretation of haematocrit with reader & centrifuge and total protein with refractometer making and staining blood smears, reticulocyte staining, systematic exam of blood smear using microscope; study of cases of anaemia
6) The WBC - lectures The 5 WBCs morphological description in health and disease. Total and differential WBC counts. WBC functions. Differences between species. How to count WBCs manually and by analyser. How analysers work. Appearance of leukemia, septicemia and WBC parasites on a blood smear
7) The WBC - tutorials Discussion / illustration of the standard operating procedures to be followed in the lab on WBCs.
8) The WBC - practicals Demo then supervised performance by each student: total count with haemocytometer, making and staining blood smear and doing a differential count measurement with microscope, systematic exam of blood smear using microscope; study of cases of anaemia, leukemia and inflammation
9) Clinical Biochemistry - lectures Functions and common Clin Biochem used for diseases of liver, kidney, gastrointestinal tract (malassimilation), pancreas (diabetes, pancreatitis, insufficiency), thyroid (feline hyperthyroidism and caninr hypothyroidism) and adrenal cortex (Cushings, Addisons). Cause of electrolyte, acid-base, and water imbalance.
10) Case Examples - lectures ClinPath profiles of marked liver disease, renal diseae, diabetes, pancreatitis, lymphoma, intestinal parasites, rodenticide poisoning including haematology, biochemistry, cytology and coagulation. Presentation and discussion of changes seen in various major and common diseases.
11) Cytology - lectures When, why and how to take and process fine needle aspirates and biopsies from lumps and bumps, body cavities, and internal masses and viscera. How to process samples and prepare slides from biopsies. The top microscopic findings in diseases lumps and bumps, lymph nodes, body cavities, joints. identifying malignancy.
12) Cytology - practicals based on the above
13) Urinalysis - lectures Why do it. How to collect, process and analyse. Gross exam, Specific gravity, Dip-stick analysis. Sediment exam for common crystals, tubular casts, blood, inflammation, and crystals. Examples of findings: renal failure, anti-freeze poisoning, urinary tract infection, urolithiasis, liver disease, cancer
14) Urinalysis - tutorials Discussion and illustration of the standard operating procedures to be followed in the lab on urinalysis
15) Urinalysis - practicals Demo then supervised performance by each student: gross description; measurement of SG with refractometer, dipstick analysis, preparation of sediment, and analysing a wet mount under the microscope. study of cases of abnormal crystals, infection, haematuria
16) Coagulation - lectures Haemostasis – blood vessel, platelet and clotting factor roles and description. Measurement of extrinsic and intrinsic clotting. Assessing fibrinolysis. History and symptoms of clotting disorders: hereditary von Willebrands, haemophilia, thrombocytopenia, rodenticide poisoning, DIC.
17) Automated haematology and clinical biochemistry analysis and profiling - Lectures on the technologies theory, use and applications
18) Automated haematology and clinical biochemistry analysis and profiling - Tutorials
19) Automated haematology and clinical biochemistry analysis and profiling - Practicals - the student will perform these analyses and generate profiles and provide a basic interpretation of them
20) Guest lectures from experts in laboratory analysis on some aspects of the above topics

Student Effort Hours: 
Student Effort Type Hours
Lectures

18

Tutorial

5

Practical

14

Autonomous Student Learning

84

Total

121

Approaches to Teaching and Learning:
Lectures
Practicals in which students have specific relevant tasks demonstrated and then perform them
Interactive Q&A tutorials
Case-based learning for recalling and integrating and applying knowledge to diagnose common diseases
Scheduled access to student laboratory given for practice and development of practical skills
Practice exams are to be worked through, with answers provided
Practical Assignment
Guest lectures from experts on laboratory analysis
Self-learning 
Requirements, Exclusions and Recommendations
Learning Exclusions:

Student cannot be awarded credit for this module if they have already achieved similar educational outcomes in higher, further or secondary education.


Module Requisites and Incompatibles
Not applicable to this module.
 
Assessment Strategy  
Description Timing Open Book Exam Component Scale Must Pass Component % of Final Grade
Assignment: Students will work in small groups and perform laboratory analyses on blood / urine samples and provide a basic interpretation. They will present their results and interpretation to the class. Throughout the Trimester n/a Graded No

10

Practical Examination: lab-based with 2 parts to it each worth 15% of the final grade: 1) Blood smears; 2) Urinalysis. Includes competency testing of specific skills learned in practicals. 1 hour End of Trimester Exam n/a Graded Yes

30

Multiple Choice Questionnaire: There will be 40 MCQs on the material covered in class. They will be equally divided across all lectures. Week 10 n/a Graded No

20

Examination: Written exam will consist of 80 MCQs and worth 40% of the final mark 1 hour End of Trimester Exam No Graded No

40


Carry forward of passed components
No
 
Resit In Terminal Exam
Spring Yes - 1 Hour
Please see Student Jargon Buster for more information about remediation types and timing. 
Feedback Strategy/Strategies

• Feedback individually to students, on an activity or draft prior to summative assessment
• Feedback individually to students, post-assessment
• Group/class feedback, post-assessment
• Online automated feedback
• Self-assessment activities

How will my Feedback be Delivered?

midterm MCQ exam results given back immediately after exam completion General comments on performance and areas of strength and weakness given to class after MCQ exam Practical performance feedback is interactive and given live during the practicals, similarly for the tutorials Practice exams with answers, and criteria for evaluation of practical skills are given

Name Role
Dr Gerald Barry Lecturer / Co-Lecturer
Ms Sandra Nicholson Lecturer / Co-Lecturer
Assoc Professor Annetta Zintl Lecturer / Co-Lecturer
Professor Finola Leonard Tutor