Learning Outcomes:
1. Describe the basic principles of immunocytochemistry and immunohistochemistry. These include, but are not limited to, the following topics:
a. Protein processing
i. Intracellular processing, secretion, extracellular matrix
b. Tissue processing
i. Fixation
ii. Embedding
iii. Sectioning
c. Antigen retrieval
d. Antibody selection
e. Colour or fluorescence-based visualisation
f. Blocking non-specific antibody binding
g. Problems with autofluorescence or endogenous peroxidase activity
h. Relevant controls
i. Assay optimisation
j. Imaging techniques
2. Demonstrate laboratory competence in performing tasks relating to Learning Outcome 1.