PHYS40100 Analysis of protein distribution in tissues - Immunohistochemistry

Academic Year 2023/2024

This module is designed to provide an introduction to antibody-based techniques for assessing protein distribution in cells (immunocytochemistry) or tissues (immunohistochemistry) with an emphasis on assay design and development. The primary aim of this module is to give students hands-on laboratory experience in immunocytochemistry and/or immunohistochemistry, and an understanding of the various controls that are necessary to ensure reliable data.

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Curricular information is subject to change

Learning Outcomes:

1. Describe the basic principles of immunocytochemistry and immunohistochemistry. These include, but are not limited to, the following topics:

a. Protein processing
i. Intracellular processing, secretion, extracellular matrix

b. Tissue processing
i. Fixation
ii. Embedding
iii. Sectioning

c. Antigen retrieval

d. Antibody selection

e. Colour or fluorescence-based visualisation

f. Blocking non-specific antibody binding

g. Problems with autofluorescence or endogenous peroxidase activity

h. Relevant controls

i. Assay optimisation

j. Imaging techniques

2. Demonstrate laboratory competence in performing tasks relating to Learning Outcome 1.

Student Effort Hours: 
Student Effort Type Hours
Tutorial

12
Laboratories

60
Autonomous Student Learning

40
Total

112
Approaches to Teaching and Learning:
This module involves approximately ten weeks of laboratory-based practical activity.
Students will work closely with a research active supervisor.
Teaching will involve laboratory work; active/task-based learning; critical writing; reflective learning; enquiry & problem-based learning.
 
Requirements, Exclusions and Recommendations

Not applicable to this module.


Module Requisites and Incompatibles
Not applicable to this module.
 
Assessment Strategy  
Description Timing Open Book Exam Component Scale Must Pass Component % of Final Grade
Continuous Assessment: Laboratory Report Varies over the Trimester n/a Graded Yes

100

Carry forward of passed components
No
 
Remediation Type Remediation Timing
Repeat Within Two Trimesters
Please see Student Jargon Buster for more information about remediation types and timing. 
Feedback Strategy/Strategies

• Feedback individually to students, post-assessment

How will my Feedback be Delivered?

After the laboratory reports have been graded individual feedback will be available.

Timetabling information is displayed only for guidance purposes, relates to the current Academic Year only and is subject to change.
 

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