MICR40070 Enzyme Technology and Protein Engineering

Academic Year 2024/2025

The aim of the course is to introduce the scientific background and the state-of-the-art strategies and methods of enzyme/protein engineering. In particular, engineering enzymes for the synthesis of value added compounds has become central to modern biotechnology. The focus of this module will be placed on evolutive methods (directed molecular evolution) and rational protein design. The topic will be addressed by lectures providing the required molecular biology, computational and chemistry background, detailed introduction into various methodologies and case studies.

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Curricular information is subject to change

Learning Outcomes:

Upon successful completion of this module, students shall be able to:
1. Understand the theory of directed evolution and rational design for enzyme engineering;
2. Evaluate a number of in vitro DNA mutation technologies;
3. Have a good understanding of different high-throughput selection and screening technologies.

Indicative Module Content:

1. Introduction to the course and enzyme technology
2. Basics of enzymes as biocatalysts
3. Enzyme discovery
4. Protein engineering: needs
5. Strategies for protein engineering I
6. Strategies for protein engineering II
7. Protein engineering case study
8. Practical limitations in protein engineering
9. Synthetic biology
10. Bioinformatic tools for protein engineering
11. Phage display technology
12. Other surface display technologies

Student Effort Hours: 
Student Effort Type Hours
Lectures

12

Specified Learning Activities

32

Autonomous Student Learning

70

Total

114

Approaches to Teaching and Learning:
lectures;
case-based learning;
active/task-based learning;
peer and group work;
student presentations 
Requirements, Exclusions and Recommendations

Not applicable to this module.


Module Requisites and Incompatibles
Equivalents:
Enzyme Technology&Protein Eng (INDM40070)


 
Assessment Strategy  
Description Timing Open Book Exam Component Scale Must Pass Component % of Final Grade
Exam (In-person): 2 hour, close book exam n/a Graded No

100


Carry forward of passed components
No
 
Remediation Type Remediation Timing
In-Module Resit Prior to relevant Programme Exam Board
Please see Student Jargon Buster for more information about remediation types and timing. 
Feedback Strategy/Strategies

• Online automated feedback

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Name Role
Dr Tanja Narancic Lecturer / Co-Lecturer
Dr Yan Yan Lecturer / Co-Lecturer