MICR40070 Enzyme Technology and Protein Engineering

Academic Year 2021/2022

The aim of the course is to introduce the scientific background and the state-of-the-art strategies and methods of enzyme/protein engineering. In particular, engineering enzymes for the synthesis of value added compounds has become central to modern biotechnology. The focus of this module will be placed on evolutive methods (directed molecular evolution) and rational protein design. The topic will be addressed by lectures providing the required molecular biology, computational and chemistry background, detailed introduction into various methodologies and case studies.

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Curricular information is subject to change

Learning Outcomes:

Upon successful completion of this module, students shall be able to:
1. Understand the theory of directed evolution and rational design for enzyme engineering;
2. Evaluate a number of in vitro DNA mutation technologies and differentiate selection and screening for directed enzyme evolution;
3. Have a good understanding of different protein/enzyme separation techniques;
4. Analyze the advantages and disadvantages of various molecular modification techniques for recombinant protein/enzyme purification;

Indicative Module Content:

1. Introduction to the course and enzyme technology
2. Basics of enzymes as biocatalysts
3. Enzyme discovery and protein engineering
4. Directed evolution of enzymes I – DNA engineering
5. Directed evolution of enzymes I – screening methods
6. Enzyme production
7. Enzyme purification
8. Oxidoreductases
9. Cofactor regeneration
10. Proteases
11. Case study
12. Review lecture, exam preparation

Student Effort Hours: 
Student Effort Type Hours
Lectures

12
Specified Learning Activities

32
Autonomous Student Learning

70
Total

114
Approaches to Teaching and Learning:
lectures;
case-based learning;
active/task-based learning;
peer and group work;
student presentations 
Requirements, Exclusions and Recommendations

Not applicable to this module.


Module Requisites and Incompatibles
Equivalents:
Enzyme Technology&Protein Eng (INDM40070)


 
Assessment Strategy  
Description Timing Open Book Exam Component Scale Must Pass Component % of Final Grade
Examination: Written exam 2 hour End of Trimester Exam No Graded No

100

Carry forward of passed components
No
 
Remediation Type Remediation Timing
In-Module Resit Prior to relevant Programme Exam Board
Please see Student Jargon Buster for more information about remediation types and timing. 
Feedback Strategy/Strategies

• Online automated feedback

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Name Role
Dr Tanja Narancic Lecturer / Co-Lecturer
Dr Yan Yan Lecturer / Co-Lecturer