CHEN40730 Animal Cell Culture Technology (PT)

Academic Year 2022/2023

This module aims to develop the student's understanding of the structure, function and behaviour of mammalian cells in culture and to highlight their importance in the production of biologics. The steps involved in both upstream and downstream cell bioprocessing will be described. Students will look at the development of production cell lines, procedures used for cell banking, and characterisation. The techniques used to monitor the growth, viability and productivity of cells during a bioprocess will be examined. The module will look in detail at the processes involved in production of therapeutic antibodies (mAbs), antibody drug conjugates (ADCs), vaccines and cell therapies.

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Curricular information is subject to change

Learning Outcomes:

On completion of this module students should be able to:

1. Describe the techniques involved in the generation of cell lines for production and to describe the procedures involved in cell banking and cell characterisation.
2. To describe the types of media formulations available for both small and large scale cell culture
3. Describe the methods and technologies used for large-scale cell culture of mammalian cells for biologic production.
4. Describe the physiological responses of animal cells to environmental conditions in culture vessels, including those arising from nutrient limitation and adverse hydrodynamic effects.
5. To understand and describe the techniques involved in monitoring growth, viability, metabolism and productivity of cells during a bioprocess.
6. Describe the upstream and downstream production of mAbs and ADCs.
7. Describe the production of vaccines in mammalian cells, both live and attenuated.
8.Describe the recent developments involved in production of cell therapies and tissue engineered constructs.

Indicative Module Content:

Overview of the production of biologics in mammalian cells
Generation of production cell lines, CHO, NS0
Physical environment, tissue culture flasks and essential equipment, laminar flows and clean rooms
Chemical environment: types of media, SF, CDM, and PFM, water quality
Large scale culture of anchorage dependent and independent cells : cell factories, microcarriers
Growth of cells in STB, CPPs
Measurement of cell viability and growth
Production of mAbs and ADCs in CHO cells: overview of both upstream and downstream processes
Production of Vaccines
Production of cell therapies
Glycoslylation and QbD

Student Effort Hours: 
Student Effort Type Hours
Autonomous Student Learning






Approaches to Teaching and Learning:
Case studies
Student presentations 
Requirements, Exclusions and Recommendations

Not applicable to this module.

Module Requisites and Incompatibles
Not applicable to this module.
Assessment Strategy  
Description Timing Open Book Exam Component Scale Must Pass Component % of Final Grade
Class Test: Short questions 2 hour End of Trimester Exam n/a Graded No


Assignment: Topic Review Coursework (End of Trimester) n/a Graded No


Assignment: Product review Week 7 n/a Graded No


Carry forward of passed components
Resit In Terminal Exam
Spring Yes - 2 Hour
Please see Student Jargon Buster for more information about remediation types and timing. 
Feedback Strategy/Strategies

• Group/class feedback, post-assessment

How will my Feedback be Delivered?

Not yet recorded.

R.I. Freshney, Animal cell culture : a practical approach. 3rd edition, Oxford University Press (5th, 2005) : earlier and later editions also suitable
Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Butler et al,
Biopharmaceuticals, Biochemistry& Biotechnology, G Walsh
Biotechnology and Biopharmaceuticals : Transforming Proteins and Genes into Drugs, Rodney JY Ho,
Encyclopedia of Industrial Biotechnology: Bioprocess, Bio-separation, and Cell Technology
Animal cell culture & technology, Michael Butler (The basics)