Learning Outcomes:
On completion of this module students should be able to:
1. Describe the general layout of an industrial biopharmaceutical facility
2. To identify the utilities and equipment required for production of biopharmaceuticals.
3. Describe the cleaning-in-place (CIP) and steam-in-place (SIP) protocols for setting up a stainless-steel bioreactor.
4. Measure the growth and viability of CHO cells manually and using an automated cell counter.
5. Measure the rate of glucose and lactate metabolism in CHO cells in culture.
6. Understand the steps involved in downstream processing of biopharmaceuticals in particular ultrafiltration and centrifugation.
7. Understand the steps involved in packing a large-scale chromatography unit.
Indicative Module Content:
Students will complete 6 experiments in NIBRT
1. Facilities
2. Cell Culture
3. Bioreactor SIP
4. Packing a large-scale chromatography column
5. Ultrafiltration
6. Disc stack centrifuge