Explore UCD

UCD Home >

CHEN30250

Academic Year 2024/2025

Biochemical Engineering Lab 2 (CHEN30250)

Subject:
Chemical Engineering
College:
Engineering & Architecture
School:
Chem & Bioprocess Engineering
Level:
3 (Degree)
Credits:
5
Module Coordinator:
Dr Roderick Jones
Trimester:
Spring
Mode of Delivery:
On Campus
Internship Module:
No
How will I be graded?
Letter grades

Curricular information is subject to change.

This is a core module for the BE in Chemical Engineering with Biochemical Engineering Minor. The purpose of the module is to provide students with theory and experience of the equipment, utilities and processes associated with a typical biopharmaceutical production facility. Practical sessions will take place during Spring in the School of Chemical Engineering, in conjunction with online training sessions facilitated by National Institute for Bioprocessing Research & Training (NIBRT) and self-directed practical elements. Students, working in pairs/groups will scheduled for a series of practical sessions, on which they will report, via technical reports and associated assignments. Activities will be selected from the following:


- introduction to the NIBRT facilities
- introduction to regulatory affairs for bioprocessing and gxp processing
- contamination control
- basic cell culture techniques, including use of laminar air flow cabinets
- upstream processing: bioreactor instrumentation & steam-in-place (SIP), disposable systems
- downstream processing: centrifugation, tangential-flow filtration (TFF), process-scale ion-exchange chromatography (equipment, column packing, asymmetry testing)
- enzyme resolution and handling techniques

A laboratory manual will be provided.
In preparing for laboratory sessions and in preparing reports and assignments, students are expected to consult the relevant literature, including industry standards and regulations and relevant CHEN module materials.

This module will also include lectures (and associated exercises) on experimental design principles and on the principles of operations/techniques of relevance to process/analytical applications.


An alternative linear mark-to-grade conversion system (40% pass) is used in this module for grades of C- and above (i.e. C-: 55.00-59.99; C: 60.00-64.99; C+65:00-69.99; B-:70.00-74.99; B: 75.00-79.99; B+: 80.00-84.99; A-: 85.00-89.99; A: 90.00-94.99; A+:95.00-100.00).


About this Module

Learning Outcomes:

On completion of this module the student should:

1. Have an applied knowledge of the equipment and utilities associated with a typical biopharmaceutical production facility.
2. Appreciate the need for aseptic technique and sterility in a typical biopharmaceutical facility.
3. Be able to identify and explain the role of the main components of a fully-instrumented bioreactor.
4. Be able to explain the nature and importance of replication in experimental design.
5. Have undertaken a complete in-place sterilization and transformation of cell experiment.
6. Be familiar with the basic techniques for working with CHO cells, for the preparation of bioreactor inocula.
7. Be familiar with large-scale chromatography equipment, including column packing and column testing for symmetry.
8. Be familiar with selected cell culture principles and applied knowledge
9. Be familiar with bioprocessing regulatory requirements and gxp processing
10. Be able to apply the principles of experimental design.

Student Effort Hours:
Student Effort Type Hours
Specified Learning Activities

10
Autonomous Student Learning

80
Lectures

8
Laboratories

24
Total

122

Approaches to Teaching and Learning:
Laboratory work; peer and group work; lectures; critical writing; student presentations, etc.

Requirements, Exclusions and Recommendations

Not applicable to this module.


Module Requisites and Incompatibles
Not applicable to this module.
 

Assessment Strategy
Description Timing Component Scale Must Pass Component % of Final Grade In Module Component Repeat Offered
Assignment(Including Essay): Assignment based on the Design of Experiment lecture content Week 9, Week 10, Week 11, Week 12 Alternative linear conversion grade scale 40% No
5
 No
Practical Skills Assessment: 5 individual reports, based upon laboratory practicals (including inclass labs, NIBRT facilitated laborites and self guided experiments) Week 2, Week 3, Week 4, Week 5, Week 6, Week 7, Week 8, Week 9, Week 10, Week 11, Week 12 Alternative linear conversion grade scale 40% No
75
 No
Quizzes/Short Exercises: Bioreaction operations assignment Week 3, Week 4, Week 5 Alternative linear conversion grade scale 40% No
5
 No
Quizzes/Short Exercises: Aseptic technique assignment Week 4, Week 5, Week 6 Alternative linear conversion grade scale 40% No
5
 No
Quizzes/Short Exercises: MCQ based upon NIBRT lecture content Week 6, Week 7, Week 8, Week 9, Week 10, Week 11 Alternative linear conversion grade scale 40% No
10
 No

Carry forward of passed components
Yes
 

Resit In Terminal Exam
Summer No
Please see Student Jargon Buster for more information about remediation types and timing. 

Feedback Strategy/Strategies

• Feedback individually to students, post-assessment
• Online automated feedback
• Peer review activities
• Self-assessment activities

How will my Feedback be Delivered?

Not yet recorded.

Name Role
Dr Colin Clarke Lecturer / Co-Lecturer
Dr Roderick Jones Lecturer / Co-Lecturer

Timetabling information is displayed only for guidance purposes, relates to the current Academic Year only and is subject to change.
Spring Laboratory Offering 1 Week(s) - 21, 22, 23, 24, 25 Tues 09:00 - 12:50
Spring Laboratory Offering 1 Week(s) - 21, 22, 23, 24, 25 Tues 14:00 - 17:50
Print this page
Fill in the following details:
Enter the body of your query here.

From time to time UCD would like to send you further information that we feel, based on your enquiry, would be of interest to you.