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Curricular information is subject to change
On completion of the protein structure part of this module students should be able to: (1) Explain the fundamentals of CD spectroscopy (2) Explain the applications of CD spectroscopy with several examples (3) Explain the importance of amino acids for formation of protein structural features commonly found in proteins (4) Explain the important structural features of the polypeptide structure (5) Explain the one dimensional and two dimensional NMR techniques for polypeptide structure determination and discuss the advanced pulse sequences and their importance to protein structure determination (6) Discuss the strategies of protein structure determination by NMR spectroscopy and explain the biophysical studies, protein-protein interactions, limitations of NMR. Protein structure analysis will cover (7) Introduction to mass spectrometry; Historical developments and overview of the emerging role of mass spectrometry in protein structure characterization (8) mass spectrometry of native proteins and of protein assemblies; Ion mobility mass spectrometry (9) Determination of protein primary sequence using de novo and database-dependent approaches, identifying and mapping protein modifications by tandem mass spectrometry (10) Studying macromolecular assemblies using mass spectrometry: Affinity purification, chemical cross-linking, deuterium exchange mass spectrometry (11) understanding of structural proteomics, chemical proteomics, and functional proteomics and (12) Integrating experimental and computational data to solve complex structural problems.
|Student Effort Type||Hours|
|Autonomous Student Learning||
It is anricipated that students will have a good understanding basic NMR spectroscopy and other analytical techniques before signing to this module.
|Resit In||Terminal Exam|
|Spring||Yes - 2 Hour|
• Feedback individually to students, post-assessment
Students are encouraged to read literature and published articles related to the course material which will be discussed in great detail. This will allow the students to be competent in structural biology techniques. This will further enhance the thinking and writing skills.
|Assoc Professor Gerard Cagney||Lecturer / Co-Lecturer|
|Lecture||Offering 1||Week(s) - 1||Fri 10:00 - 10:50|
|Lecture||Offering 1||Week(s) - 2, 3, 4||Fri 10:00 - 10:50|
|Lecture||Offering 1||Week(s) - 1, 2, 3, 4||Mon 09:00 - 09:50|
|Lecture||Offering 1||Week(s) - 1, 2, 3, 4||Wed 09:00 - 09:50|