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BIOC30030

Academic Year 2024/2025

Biochemist's Toolkit (BIOC30030)

Subject:
Biochemistry
College:
Science
School:
Biomolecular & Biomed Science
Level:
3 (Degree)
Credits:
5
Module Coordinator:
Assoc Professor Yan Yan
Trimester:
Spring
Mode of Delivery:
Blended
Internship Module:
No
How will I be graded?
Letter grades

Curricular information is subject to change.

Introduction to the techniques and methods required for a modern biochemical approach to the problems of biology. These include biochemical assays, separation techniques and biomolecule analysis. The theory and practice of: spectrophotometry and fluorescence techniques, clinical biochemistry analysis, centrifugation, protein and nucleic acid isolation and chromatographic separation techniques. Analytical techniques such as SDS-electrophoresis, immunoblotting and mass spectrometry. The application of recombinant DNA technology with an emphasis on the generation of fusion and epitope-tagged proteins to help in the identification and purification of the expressed proteins. Molecular biology and cell biology techniques including cloning, gene silencing, flow cytometry and cell imaging.

About this Module

Learning Outcomes:

On completion of this module students should be able to:1. Analyse biological systems at the molecular, cellular levels as well as organs and whole animals. 3. Use spectrophotometric and fluorescence techniques to analyse and quantify biomolecules. 4. Develop strategies for protein purification and analyse biomolecules by electrophoretic techniques. 5.Utilize radioisotope methods for examining biological systems. 6.Understand how electron microscopy, confocal microscopy, light and fluorescence spectroscopy can be used to study biological tissues. 7.Know how to use preparative and analytical centrifugation procedures. 8. Understand the basis for a range of clinical biochemistry tests. 8. Explain how gene silencing techniques are used to deplete proteins in cell and animal studies.

Student Effort Hours:
Student Effort Type Hours
Specified Learning Activities

8
Autonomous Student Learning

66
Lectures

23
Practical

15
Total

112

Approaches to Teaching and Learning:
Lectures; reflective learning; labwork; enquiry & problem-based learning

Requirements, Exclusions and Recommendations
Learning Recommendations:

It is recommended that students taking this module have successfully completed BMOL20110 and BIOC20060.


Module Requisites and Incompatibles
Not applicable to this module.
 

Assessment Strategy
Description Timing Component Scale Must Pass Component % of Final Grade In Module Component Repeat Offered
Exam (In-person): 2 hour close book exam
short essays		 End of trimester
Duration:
2 hr(s)
 Graded No
70
 No
Practical Skills Assessment: practical assessment includes dry and wet lab experiments. Week 10 Graded No
30
 No

Carry forward of passed components
Yes
 

Resit In Terminal Exam
Autumn Yes - 2 Hour
Please see Student Jargon Buster for more information about remediation types and timing. 

Feedback Strategy/Strategies

• Feedback individually to students, post-assessment

How will my Feedback be Delivered?

Not yet recorded.

Name Role
Dr Jonathan Bailey Lecturer / Co-Lecturer
Dr Patrick Caffrey Lecturer / Co-Lecturer
Dr Jens Rauch Lecturer / Co-Lecturer
Assoc Professor Yan Yan Lecturer / Co-Lecturer
Yuri Babyuk Tutor
Master Ciaran Carey Tutor
Darragh Doherty Tutor
Ms Elena Giardini Tutor
Kameshwari Tata Tutor

Timetabling information is displayed only for guidance purposes, relates to the current Academic Year only and is subject to change.
Spring Practical Offering 1 Week(s) - 21, 24 Mon 10:00 - 12:50
Spring Practical Offering 1 Week(s) - 26 Mon 10:00 - 12:50
Spring Practical Offering 1 Week(s) - 29 Mon 10:00 - 12:50
Spring Practical Offering 1 Week(s) - 32 Mon 10:00 - 12:50
Spring Lecture Offering 1 Week(s) - 20, 21, 22, 23, 24, 25, 26, 29, 30, 31, 32, 33 Thurs 16:00 - 16:50
Spring Lecture Offering 1 Week(s) - 20, 21, 22, 23, 24, 25, 26, 29, 30, 31, 32, 33 Tues 15:00 - 15:50
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